ABSTRACT
<p><b>AIM</b>To investigate the chemical constituents of water-soluble part of stem of Nauclea officinalis.</p><p><b>METHODS</b>The compounds were isolated and purified by resins, silica gel, and Sephadex LH20 column chromatography repeatedly, and their structures were identified by spectral analysis.</p><p><b>RESULTS</b>Seven compounds were isolated and identified as: naucleamide A-10-O-beta-D-glucopyranoside (I), 5-beta-carboxystrictosidin (II), sweroside (III), loganin (IV), 3,4-dimethoxyphenyl-beta-D-glucopyranoside (V), 3, 4, 5-trimethoxyphenyl-beta-D-glucopyranoside (VI), 2-phenethylrutinoside (VII).</p><p><b>CONCLUSION</b>Compound I is a new indole alkaloid glucoside, compounds I-VII were isolated from this plant for the first time.</p>
Subject(s)
Carbolines , Chemistry , Glucosides , Chemistry , Glycosides , Chemistry , Indole Alkaloids , Chemistry , Iridoid Glucosides , Iridoids , Chemistry , Molecular Conformation , Molecular Structure , Plant Stems , Chemistry , Plants, Medicinal , Chemistry , Rubiaceae , ChemistryABSTRACT
Objective: To investigate the constituents of indole alkaloids in Ervatamia yunnanensis and their antiaddictive bioactivities. Methods: The preliminary alkaloids (TEYA) were extracted by 95 % EtOH from the stems of Ervatamia yunnanensis. TEYA was then purified by silica gel and Sephadex LH20 column chromatography repeatedly and its structure was identified with spectral analysis. The antiaddictive effects of these indole alkaloids were evaluated by Conditioned Place Preference (CPP) experiments. Results: Nine indole alkaloids were obtained and identified as: coronaridine (1) , voacangine (2), 3-R-ethoxycoronaridine (3), 3-S-ethoxycoronaridine (4), 19-epi-heyneanine (5), heyneanine (6), 19-epi-voacristine (7), coronaridine hydroxyindolenine (8) and 12-methoxyl-voaphylline (9). Among them voacangine (2) and 19-epi-voacristine (7) were effective in prevention and treatment of addiction induced by morphine in rats (n=8). Conclusion: Compounds 3, 4, 8, and 9 have been isolated from Ervatamia yunnanensis for the first time and coronaridine-type indole alkaloids are the main antiaddictive constituents of E. yunnanensis.
ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Ervatamia hainanensis.</p><p><b>METHOD</b>The compounds were separated and purified by column chromatography with silica gel, and identified by IR, MS, NMR and 2D-NMR.</p><p><b>RESULT</b>Five compounds were identified as I (isolariciresinol 9-O-beta-D-glucopyranoside), II (cycloartenol), III (beta-amyrin acetate), IV (beta-sitosterol), V (daucosterol), respectively.</p><p><b>CONCLUSION</b>All the compounds were isolated from this plant for the first time.</p>